
Transfer the powdered tissue to a clean Eppendorf tube with 100 ul of extraction buffer mix as prepared above (adding a little extraction buffer mix to the tube helps with the transfer).
Phenol-Chloroform-Isoamyl Alcohol DNA Extraction - protocols.io
Jun 11, 2025 · This protocol is for an organic isolation method of DNA from environmental DNA samples preserved in filter membranes. This protocol is focused on the acquisition of long fragments. 1L of …
Genomic DNA purification protocol for whole genome sequencing …
Jan 14, 2025 · This protocol outlines the steps for genomic DNA extraction and purification from bacterial samples, including an enhanced bead-beating method for improved DNA yield and quality.
Extract-N-Amp Equivalent DNA Extraction Protocol
Jun 19, 2025 · Abstract This extraction protocol uses an Extract-N-Amp equivalent solution to quickly and cheaply perform DNA extractions. This protocol has been widely used for dried specimens of …
This protocol for DNA isolation is universal for most biological specimens. The method will effectively isolate DNA from whole blood, bones, plant samples, soil, herbarium, the mycelium of fungi, and …
DNA extraction (Salting out) - protocols.io
Incubate in a water bath at 55°C for approximately 1:30h or overnight; Add 300µL of NaCl (5M) and centrifuge for 10min at 10.000rpm. Transfer supernatant containing the DNA to microcentrifuge tube …
Bacterial and fungal DNA extraction protocol for long-read whole …
Sep 26, 2024 · As DNA extraction is a crucial step prior to obtain high-quality genomes, we developed a two-days DNA extraction protocol efficient for the extraction of High-Molecular-Weight (HMW) …
Abstract This protocol explains the Standard Operating Protocol for manually extracting DNA using Gentra Puragene.
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DNA Extraction from Biopsy Samples (Ph:Chl & Microcon) - protocols.io
Jul 18, 2025 · This protocol describes our latest protocol for extracting DNA from standard biopsy samples of whale skin. It uses a combination of phenol:chloroform to separate the DNA from other …